cardiotomskСибирский журнал клинической и экспериментальной медициныSiberian Journal of Clinical and Experimental Medicine2713-29272713-265XTSU publishing10.29001/2073-8552-2023-38-2-147-155cardiotomsk-1799Research ArticleКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯCLINICAL STUDIESПатогенетическая значимость полиморфных вариантов гена TREM-1 в развитии полиорганной недостаточности после кардиохирургического вмешательстваPathogenetic significance of polymorphic variants in the TREM-1 gene in the multiple organ failure risk after cardiac surgeryhttps://orcid.org/0000-0002-9714-4080ХуторнаяМ. В.KhutornayaM. V.

Хуторная Мария Владимировна - младший научный сотрудник, лаборатория геномной медицины, отдел экспериментальной медицины

650002, Российская Федерация, Кемерово, Сосновый бульвар, 6 

Mariya V. Khutornaya - Junior Research Scientist, Laboratory of Genomic Medicine, Department of Experimental Medicine

 6, Sosnovy blvd., Kemerovo, 650002, Russian Federation 

masha_hut@mail.ruhttps://orcid.org/0000-0002-4467-8732СиницкаяА. В.SinitskayaA. V.

Синицкая Анна Викторовна - канд. биол. наук, научный сотрудник, лаборатория геномной медицины, отдел экспериментальной медицины

 650002, Российская Федерация, Кемерово, Сосновый бульвар, 6 

 Anna V. Sinitskaya - Research Scientist, Laboratory of Genomic Medicine, Department of Experimental Medicine

 6, Sosnovy blvd., Kemerovo, 650002, Russian Federation 

cepoav1991@gmail.comhttps://orcid.org/0000-0002-4824-2418СиницкийМ. Ю.SinitskyM. Yu.

Синицкий Максим Юрьевич - канд. биол. наук, старший научный сотрудник, лаборатория геномной медицины, отдел экспериментальной медицины

650002, Российская Федерация, Кемерово, Сосновый бульвар, 6 

Maxim Yu. Sinitsk - Cand. Sci. (Biol.), Senior Research Scientist, Laboratory of Genomic Medicine, Department of Experimental Medicine 

 6, Sosnovy blvd., Kemerovo, 650002, Russian Federation 

sinitsky.maxim@gmail.comhttps://orcid.org/0000-0002-3002-2863ПонасенкоА. В.PonasenkoA. V.

Понасенко Анастасия Валериевна - канд. мед. наук, заведующий лабораторией геномной медицины, отдел экспериментальной медицины

 650002, Российская Федерация, Кемерово, Сосновый бульвар, 6 

Anastasia V. Ponasenko - Cand. Sci. (Med.), Head of Laboratory of Genomic Medicine, Department of Experimental Medicine 

 6, Sosnovy blvd., Kemerovo, 650002, Russian Federation 

ponaav@kemcardio.ruhttps://orcid.org/0000-0001-8370-3083ГригорьевЕ. В.GrigorievE. V.

Григорьев Евгений Валерьевич - д-р мед. наук, профессор РАН, заместитель директора по научной и лечебной работе

 650002, Российская Федерация, Кемерово, Сосновый бульвар, 6 

Evgeniy V. Grigoriev - Dr. Sci. (Med.), Deputy Director for Scientific and Medical Work 

 6, Sosnovy blvd., Kemerovo, 650002, Russian Federation 

grigev@kemcardio.ruНаучно-исследовательский институт комплексных проблем сердечно-сосудистых заболеванийРоссияFederal State Budgetary Scientific Institution Research Institute for Complex Issues of Cardiovascular DiseasesRussian Federation202307072023382147155Copyright © Хуторная М.В., Синицкая А.В., Синицкий М.Ю., Понасенко А.В., Григорьев Е.В., 20232023Хуторная М.В., Синицкая А.В., Синицкий М.Ю., Понасенко А.В., Григорьев Е.В.Khutornaya M.V., Sinitskaya A.V., Sinitsky M.Y., Ponasenko A.V., Grigoriev E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sibjcem.ru/jour/article/view/1799

Введение. На сегодняшний день существует потребность в поиске высокоспецифичного, высокочувствительного и простого в использовании маркера полиорганной недостаточности (ПОН), который поможет облегчить ранний прогноз данного неблагоприятного состояния, предотвратить осложнения и снизить смертность в раннем послеоперационном периоде.Цель: оценить патогенетическую значимость полиморфных вариантов гена TREM-1 в развитии ПОН у пациентов с ишемической болезнью сердца (ИБС) после кардиохирургического вмешательства.Материал и методы. Для проведения исследования отобраны 592 пациента с ИБС (564 пациента с неосложненным послеоперационным периодом и 28 пациентов с развившейся ПОН). Проведено генотипирование восьми полиморфных вариантов TREM-1 (rs1817537, rs3804277, rs6910730, rs7768162, rs2234246, rs4711668, rs9471535, rs2234237) методом полимеразной цепной реакции.Результаты. Выявлено, что с повышенным риском развития ПОН после планового оперативного вмешательства ассоциированы аллели Т rs2234246, G rs1817537 и Т rs3804277 TREM-1 по доминантной модели наследования. Со сниженным риском развития ПОН обнаружены ассоциации по двум полиморфным локусам TREM-1 rs7768162 и rs4711668 по аддитивной модели наследования. При изучении полиморфных вариантов rs2234237, rs6910730 и rs9471535 в отношении развития ПОН значимых отличий не выявлено. В исследовании не установлены статистически значимые связи между наследуемым гаплотипом и риском развития ПОН. При помощи программы MDR определены 3 наиболее значимые модели ген-генных взаимодействий полиморфных локусов TREM-1, ассоциированных с развитием ПОН у пациентов после кардиохирургического вмешательства.Заключение. Полученные результаты демонстрируют значимый вклад полиморфных вариантов гена TREM-1 в развитие ПОН у пациентов кардиохирургического профиля.

Introduction. Searching of highly specific, sensitive and easy-to-use markers of multiple organ failure (MOF) that will help to the early prognosis of this unfavorable condition, prevent complications and reduce mortality in the early postoperative period is very urgent for the modern medicine.Aim: To study the pathogenetic significance of the TREM-1 gene polymorphism in MOF in patients with coronary artery disease (CAD) in the early postoperative period.Material and methods. 592 CAD patients (564 patients with uncomplicated postoperative period and 28 MOF patients) were selected for the presented study. Genotyping polymorphic variants rs1817537, rs3804277, rs6910730, rs7768162, rs2234246, rs4711668, rs9471535 and rs2234237 in the TREM-1 gene was carried out by polymerase chain reaction.Results. It was found that the allele T (rs2234246), the allele G (rs1817537) and the allele T (rs3804277) in the TREM-1 gene were associated with an increased MOF risk after elective surgery according to the dominant inheritance model. TREM-1 polymorphic loci rs7768162 and rs4711668 were associated with a decreased MOF risk according to the additive inheritance model. We found no significant associations between polymorphic variants rs2234237, rs6910730, rs9471535, as well as inherited haplotype and MOF risk. Using the MDR analysis, three most significant models of gene-gene interactions of TREM-1 polymorphic loci associated with MOF risk in patients after cardiac surgery were identified.Conclusion. The obtained results demonstrate a significant contribution of polymorphic variants in the TREM-1 gene to the development of MOF in patients undergoing cardiac surgery.

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The authors declare that there are no conflicts of interest present.