cardiotomskСибирский журнал клинической и экспериментальной медициныSiberian Journal of Clinical and Experimental Medicine2713-29272713-265XTSU publishing10.29001/2073-8552-2025-40-4-123-130cardiotomsk-2917Research ArticleКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯCLINICAL STUDIESАссоциация полиморфизмов C7028T, G3010A, G9055A митохондриальной ДНК и тяжести течения хронической сердечной недостаточности ишемического генезаAssociation of mitochondrial DNA C7028T, G3010A, G9055A polymorphisms and the severity of chronic heart failure of ischemic genesishttps://orcid.org/0000-0001-7361-2161МуслимоваЭ. Ф.MuslimovaE. F.

Муслимова Эльвира Фаритовна - канд. мед. наук, научный сотрудник, лаборатория молекулярно-клеточной патологии и генодиагностики, НИИ кардиологии Томского НИМЦ.

634012, Томск, ул. Киевская, 111а

Elvira F. Muslimova - Cand. Sci. (Med.), Research Scientist, Laboratory of Molecular and Cellular Pathology and Gene Diagnostics, Cardiology Research Institute, Tomsk NRMC.

634012, Tomsk, Kievskaya str., 111a

muslimovef@yandex.ruhttps://orcid.org/0000-0002-8070-2234КужелеваЕ. А.KuzhelevaE. A.

Кужелева Елена Андреевна - канд. мед. наук, старший научный сотрудник, отделение патологии миокарда, НИИ кардиологии Томского НИМЦ.

634012, Томск, ул. Киевская, 111а

Elena A. Kuzheleva - Cand. Sci. (Med.), Senior Research Scientist, Department of Myocardial Pathology, Cardiology Research Institute, Tomsk NRMC.

634012, Tomsk, Kievskaya str., 111a

kea@cardio-tomsk.ruhttps://orcid.org/0000-0002-9488-6900ГарганееваА. А.GarganeevaA. A.

Гарганеева Алла Анатольевна - д-р мед. наук, профессор, заведующий отделением патологии миокарда, НИИ кардиологии Томского НИМЦ.

634012, Томск, ул. Киевская, 111а

Alla A. Garganeeva - Dr. Sci. (Med.), Professor, Head of the Department of Myocardial Pathology, Cardiology Research Institute, Tomsk NRMC.

634012, Tomsk, Kievskaya str., 111a

aag@cardio-tomsk.ruhttps://orcid.org/0000-0001-6066-3998АфанасьевС. А.AfanasievS. A.

Афанасьев Сергей Александрович - д-р мед. наук, профессор, заведующий лабораторией молекулярно-клеточной патологии и генодиагностики, НИИ кардиологии Томского НИМЦ.

634012, Томск, ул. Киевская, 111а

Sergey A. Afanasiev - Dr. Sci. (Med.), Professor, Head of the Laboratory of Molecular and Cellular Pathology and Gene Diagnostics, Cardiology Research Institute, Tomsk NRMC.

634012, Tomsk, Kievskaya str., 111a

tursky@cardio-tomsk.ruНаучно-исследовательский институт кардиологии, Томский национальный исследовательский медицинский центр Российской академии наук (НИИ кардиологии Томского НИМЦ)РоссияCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences (Cardiology Research Institute, Tomsk NRMC)Russian Federation202512122025404123130Copyright © Муслимова Э.Ф., Кужелева Е.А., Гарганеева А.А., Афанасьев С.А., 20252025Муслимова Э.Ф., Кужелева Е.А., Гарганеева А.А., Афанасьев С.А.Muslimova E.F., Kuzheleva E.A., Garganeeva A.A., Afanasiev S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sibjcem.ru/jour/article/view/2917Введение

Введение. Распространенные полиморфизмы митохондриальной ДНК (мтДНК) могут влиять на интенсивность клеточного дыхания и продукцию активных форм кислорода. Избыточное количество активных форм кислорода приводит к окислительному стрессу, который способствует развитию многофакторных заболеваний. Можно ожидать, что полиморфизмы мтДНК могут выступить как кандидатные локусы риска развития или прогрессирования сердечно-сосудистой патологии.

Цель исследования

Цель исследования: оценка ассоциации полиморфизмов мтДНК C7028T, G3010A и G9055A с тяжестью течения хронической сердечной недостаточности (ХСН) у пациентов с ишемической болезнью сердца (ИБС).

Материал и методы

Материал и методы. В выборку включены 97 пациентов в возрасте 63 (58; 68) лет. Перенесенный в анамнезе инфаркт миокарда (ИМ) диагностирован у 74 (76,3%) пациентов. Выполнены стандартные клинико-инструментальные методы исследования. Определены полиморфизмы мтДНК с помощью полимеразной цепной реакции (ПЦР) с последующим анализом полиморфизма длин рестрикционных фрагментов.

Результаты

Результаты. Выявлено, что среди пациентов с умеренно сниженной фракцией выброса (ФВ) аллель 7028T встречался в 2 раза чаще, чем среди пациентов с сохраненной и низкой ФВ (78,9 против 34,3% и 34,9%, p = 0,002). При низкой ФВ среди пациентов с дилатацией правого предсердия (ПП) частота аллеля 7028С составила 8 (44,4%), аллеля 7028T – 10 (55,6%); без дилатации – 20 (80,0%) и 5 (20,0%) (p = 0,024). Отсутствовала ассоциация между полиморфизмом G3010A и параметрами, характеризующими тяжесть течения ХСН. Но частота аллеля 3010A была меньше среди пациентов с потребностью в диуретической терапии, чем среди лиц, не принимавших диуретики (8,6 против 30,8%, p = 0,005). Только у 3 (3,1%) больных установлен аллель 9055A.

Заключение

Заключение. Среди пациентов с ХСН ишемического генеза выявлена ассоциация полиморфизма C7028T мтДНК с фенотипом ХСН с умеренно сниженной ФВ левого желудочка (ЛЖ), при низкой ФВ – с дилатацией ПП. Полиморфизм G3010A мтДНК продемонстрировал ассоциацию с частотой применения диуретических препаратов.

Introduction

Introduction. Common mitochondrial DNA (mtDNA) polymorphisms can affect the intensity of cellular respiration and the production of reactive oxygen species. Excessive amounts of reactive oxygen species lead to oxidative stress, which contributes to the development of multifactorial diseases. It can be expected that mtDNA polymorphisms can act as candidate risk loci for the development or progression of cardiovascular pathology.

Aim

Aim: To evaluate the association of mtDNA polymorphisms C7028T, G3010A and G9055A with the severity of chronic heart failure (CHF) in patients with ischemic heart disease.

Material and Methods

Material and Methods. The sample included 97 patients aged 63 (58; 68) years. A history of myocardial infarction was diagnosed in 74 (76.3%) patients. Standard clinical and instrumental research methods were performed. The mtDNA polymorphisms were determined using polymerase chain reaction followed by restriction fragment length polymorphism analysis.

Results

Results. It was found that among patients with a moderately reduced ejection fraction, the 7028T allele was found 2 times more often than among patients with preserved and reduced ejection fraction (EF) (78.9% versus 34.3% and 34.9%, p = 0.002). In patients with low EF and right atrial dilation, the frequency of the 7028C allele was 8 (44.4%), the 7028T allele – 10 (55.6%); without dilation – 20 (80.0%) and 5 (20.0%) (p = 0.024). There was no association between the G3010A polymorphism and parameters characterizing the severity of CHF. However, the frequency of 3010A substitution was lower among patients requiring diuretic therapy than among those not taking diuretics (8.6% vs. 30.8%, p = 0.005). Only 3 patients (3.1%) were identified with the 9055A allele.

Conclusion

Conclusion. Among patients with CHF of ischemic genesis, an association of mtDNA C7028T polymorphism with a heart failure phenotype with a moderately reduced left ventricular EF and in group with low EF with right atrial dilation was revealed. The mtDNA G3010A polymorphism was associated with a diuretic prescription.

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The authors declare that there are no conflicts of interest present.