cardiotomskСибирский журнал клинической и экспериментальной медициныSiberian Journal of Clinical and Experimental Medicine2713-29272713-265XTSU publishing10.29001/2073-8552-2019-34-3-87-96cardiotomsk-817Research ArticleКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯCLINICAL STUDIESВзаимосвязь полиморфизма I/D гена ангиотензинпревращающего фермента с формированием эссенциальной артериальной гипертензииAssociation of the I/D polymorphism of angiotensinconverting enzyme gene with the development of essential hypertensionhttps://orcid.org/0000-0002-1397-0108ПавловаО. С.PavlovaO. S.

канд. мед. наук, заведующий лабораторией артериальной гипертензии,

220036, Минск, ул. Р. Люксембург, 110Б

Cand. Sci. (Med.), Head of the Laboratory of Arterial Hypertension,

110, Luxemburg str., Minsk, 220036

olga.s_pavlova@yahoo.comhttps://orcid.org/0000-0003-2612-1823ОгурцоваС. Э.OgurtsovaS. E.

канд. биол. наук, заведующий лабораторией фармакогенетики,

220141, Минск, ул. Купревича, 5/2

Cand. Sci. (Biol.), Head of the Laboratory of Pharmacogenetics,

5/2, Kuprevicha str., Minsk, 220141

svetaogurtsova2011@mail.ruhttps://orcid.org/0000-0002-6398-7063ЛивенцеваМ. М.LiventsevaM. M.

канд. мед. наук, ведущий научный сотрудник лаборатории артериальной гипертензии,

220036, Минск, ул. Р. Люксембург, 110Б

Cand. Sci. (Med.), Principal Researcher, Laboratory of Arterial Hypertension,

110, Luxemburg str., Minsk, 220036

mari.mml@mail.ruhttps://orcid.org/0000-0001-5545-3486ЛакоткоТ. Г.LakotkoT. H.

канд. мед. наук, старший преподаватель 2-й кафедры внутренних болезней,

230009, Гродно, ул. Горького, 80

Cand. Sci. (Med.), Senior Lecturer, 2nd Department of Internal Diseases, 

80, Gorkogo str., Grodno, 230009

laktan@mail.ruhttps://orcid.org/0000-0001-9715-0475КоробкоИ. Ю.KorobkoI. Y.

канд. мед. наук, ведущий научный сотрудник лаборатории артериальной гипертензии, 

220036, Минск, ул. Р. Люксембург, 110Б

Cand. Sci. (Med.), Principal Researcher, Laboratory of Arterial Hypertension,

110, Luxemburg str., Minsk, 220036

lab.hypertension@yandex.byhttps://orcid.org/0000-0002-8244-2747ШишкоВ. И.ShyshkoV. I.

канд. мед. наук, доцент, проректор по лечебной работе,

230009, Гродно, ул. Горького, 80

Cand. Sci. (Med.), Associate Professor, Vice-Rector for Clinical Work,

80, Gorkogo str., Grodno, 230009

vshyshko@mail.ruhttps://orcid.org/0000-0002-2484-1723МрочекА. Г.MrochekA. G.

д-р мед. наук, профессор, академик Национальной академии наук Беларуси, директор,

220036, Минск, ул. Р. Люксембург, 110Б

Dr. Sci. (Med.), Professor, Academician of the Belarusian National Academy of Sciences, Director,

110, Luxemburg str., Minsk, 220036

director@cardio.byРеспубликанский научно-практический центр «Кардиология»БеларусьRepublican Scientific and Practical Centre «Cardiology»BelarusИнститут биоорганической химии Национальной Академии наук БеларусиБеларусьInstitute of Bioorganic Chemistry of National Academy of SciencesBelarusРеспубликанский научно-практический центр «Кардиология»,БеларусьRepublican Scientific and Practical Centre «Cardiology»BelarusГродненский государственный медицинский университетБеларусьGrodno State Medical UniversityBelarus2019021120193438796Copyright © Павлова О.С., Огурцова С.Э., Ливенцева М.М., Лакотко Т.Г., Коробко И.Ю., Шишко В.И., Мрочек А.Г., 20192019Павлова О.С., Огурцова С.Э., Ливенцева М.М., Лакотко Т.Г., Коробко И.Ю., Шишко В.И., Мрочек А.Г.Pavlova O.S., Ogurtsova S.E., Liventseva M.M., Lakotko T.H., Korobko I.Y., Shyshko V.I., Mrochek A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sibjcem.ru/jour/article/view/817Цель исследования

Цель исследования: определение влияния полиморфизма I/D гена ангиотензинпревращающего фермента (АСЕ) на развитие эссенциальной артериальной гипертензии (АГ) с учетом гендерных различий.

Материал и методы

Материал и методы. Проведена оценка клинических данных и молекулярно-генетическое исследование у 602 человек, из них у 401 пациента с эссенциальной АГ и 201 человека из группы контроля, представляющих белорусскую этническую группу. Генотипирование выполняли с помощью метода полимеразной цепной реакции и полиморфизма длин рестрикционных фрагментов.

Результаты

Результаты. Распределение генотипов полиморфизма I/D гена АСЕ не различалось между пациентами с АГ и нормотензивными лицами – генотипы II, ID, DD были выявлены у 100 (24,9%), 192 (47,9%), 109 (27,2%) больных и у 52 (25,9%), 108 (53,7%), 41 (20,4%) человек из группы контроля соответственно. Получены отличия по распределению генотипа DD у мужчин с АГ и группой контроля, где встречаемость составила 28,4 и 17,3% (р  =  0,04) в отличие от женщин – 25,8 и 23,3% (р  =  0,64). Hосительство генотипа DD у мужчин в сравнении с ID и DD (рецессивная модель) полиморфизма I/D гена ACE увеличивало вероятность развития АГ в 1,9 раза (ОШ  =  1,89; 95% ДИ  =  1,043,44). При анализе распространенности факторов риска в зависимости от полиморфизма I/D гена АСЕ выявлено, что у больных мужчин с генотипом DD отягощенная наследственность по развитию преждевременных сердечно-сосудистых заболеваний встречалась чаще – у 23 (37,7%) человек в отличие от генотипов II и ID – у 13 (21,7%) и 14 (14,9%) человек (χ2   =  1,16; р  =  0,005) и преимущественно по отцовской линии.

Выводы

Выводы. Формирование АГ ассоциировано с носительством мутантного генотипа DD полиморфизма I/D гена АСЕ у мужчин. 

Objective

Objective. To determine the impact of the I/D polymorphism of the angiotensin-converting enzyme (ACE) gene on the development of essential hypertension, taking into account gender differences.

Material and Methods

Material and Methods. Clinical data were assessed and a molecular genetic study was performed in 602 people including 401 patients with essential hypertension and 201 individuals of the control group, representing the Belarusian ethnic group. Genotyping was performed using the method of polymerase chain reaction and restriction fragment length polymorphism.

Results

Results. The distribution of genotypes of the I/D polymorphism of the ACE gene did not differ between patients with hypertension and normotensive individuals: II, ID, and DD genotypes were detected in 100 (24.9%), 192 (47.9%), and 109 (27.2%) patients and in 52 (25.9%), 108 (53.7%), and 41 (20.4%) people of the comparison group, respectively. Differences were found between the distribution of DD genotype in men with hypertension and in the control group, where the frequencies were 28.4% and 17.3% (p  =  0.04), respectively, in contrast to no differences in women: 25.8% and 23.3% (p  =  0.64), respectively. Carrying the DD genotype in men compared with the ID and DD genotypes (recessive model) of the I/D polymorphism of the ACE gene increased the probability of developing essential hypertension by 1.9 times (OR   =   1.89; 95% CI  =  1.04-3.44). The analysis of the prevalence of risk factors depending on the I/D polymorphism of the ACE gene showed that male patients with the DD genotype more often had burdened heredity in regard to the development of premature cardiovascular diseases (23 patients (37.7%)) compared with the individuals with II and ID genotypes: 13 (21.7%) and 14 (14.9%) patients, respectively (χ2  =  1.16; p  =   0.005), and mainly through the paternal line.

Conclusions

Conclusions. Development of essential hypertension is associated with the carriership of the mutant DD genotype of I/D polymorphism of the ACE gene in men. 

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The authors declare that there are no conflicts of interest present.