cardiotomskСибирский журнал клинической и экспериментальной медициныSiberian Journal of Clinical and Experimental Medicine2713-29272713-265XTSU publishing10.29001/2073-8552-2020-35-2-163-166cardiotomsk-991Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL STUDIESРоль β-адренорецепторов в стресс-индуцированном повреждении сердца у крыс в модели синдрома такотсубоThe role of β-adrenergic receptors in stress-induced cardiac injury in a rat model of Takotsubo syndromehttps://orcid.org/0000-0001-9603-822XКурбатовБ. К.KurbatovB. K.

младший научный сотрудник, лаборатория экспериментальной кардиологии,

634012, Томск, ул. Киевская, 111а

Junior Research Scientist, Laboratory of Experimental Cardiology,

111a, Kievskaya str., Tomsk, 634012

bobersanker@gmail.comhttps://orcid.org/0000-0002-1991-6516ПрокудинаЕ. С.ProkudinaE. S.

канд. мед. наук, научный сотрудник, лаборатория экспериментальной кардиологии,

634012, Томск, ул. Киевская, 111а

Cand. Sci. (Med.), Research Scientist, Laboratory of Experimental Cardiology,

111a, Kievskaya str., Tomsk, 634012

goddess27@mail.ruНаучно-исследовательский институт кардиологии, Томский национальный исследовательский медицинский центр Российской академии наукРоссияCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesRussian Federation202010072020352163166Copyright © Курбатов Б.К., Прокудина Е.С., 20202020Курбатов Б.К., Прокудина Е.С.Kurbatov B.K., Prokudina E.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sibjcem.ru/jour/article/view/991Актуальность

Актуальность. На данный момент неясен патофизиологический механизм реализации повреждения сердца при синдроме такотсубо. Остается неясна роль отдельных субтипов адренорецепторов (АР) в реализации стресс-индуцированного повреждения сердца (СИПС).

Цель

Цель: оценить роль β-АР в реализации СИПС. Материал и методы. Исследование было выполнено на 84 крысах-самках линии Вистар. Для моделирования СИПС крысы подвергались 24-часовой иммобилизации в положении на спине. Для определения степени повреждения сердца использовали радиофармпрепарат 99mTc-пирофосфат.

Результаты

Результаты. Установлено, что блокада β-АР (-)-пропранололом уменьшает степень повреждения сердца на 38,4%. Показано, что селективные блокаторы β1 -АР атенолол и небиволол уменьшают степень аккумуляции 99mTc-пирофосфата в сердце в 2 и 2,55 раза соответственно. Блокада β2 -АР селективным блокатором ICI-118,551 вызывает повышение степени аккумуляции 99mTc-пирофосфата в сердце на 34,6%. Селективный блокатор β3 -АР L-748337 не оказывает эффекта на аккумуляцию 99mTc-пирофосфата в сердце.

Выводы

Выводы. β1 -АР участвуют в повреждающем действии стресса на сердце при иммобилизации. β2 -АР обладают кардиопротекторным эффектом при иммобилизации. β3 -АР не играют существенной роли в стресс-индуцированном повреждении сердца при однократном действии стрессора. 

Introduction

Introduction. The pathophysiological mechanisms of cardiac injury in Takotsubo syndrome are currently poorly understood. The role of adrenergic receptor (AR) subtypes in the development of stress-induced myocardial injury (SIMI) remains unclear.

Aim

Aim. To assess the role of β-ARs in the development of SIMI.

Material and Methods

Material and Methods. The study was performed using female Wistar rats (n = 84). Rats were subject to 24-hour immobilization in the supine position to simulate SIMI. 99mTc-pyrophosphate radiopharmaceutical was used to determine the degree of cardiac injury.

Results

Results. The study showed that β-AR blockade with propranolol reduced the degree of cardiac injury by 38.4%. Selective β1- AR antagonists, atenolol and nebivolol, led to 2.00- and 2.55-fold decreases in 99mTc pyrophosphate accumulation in the heart, respectively. Blockade of β2-ARs by a selective antagonist ICI-118.551 caused an increase in the degree of 99mTc-pyrophosphate accumulation in the heart by 34.6%. A selective β3-AR antagonist L-748337 did not affect 99mTc pyrophosphate accumulation in the heart.

Conclusions

Conclusions. The study showed that β1-ARs are involved in the damaging effects of stress on the heart during immobilization stress. β2-AR had a cardioprotective effect in immobilization. β3-AP did not play a significant role in the stress-induced cardiac injury with a single exposure to the stressor. 

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The authors declare that there are no conflicts of interest present.