The role of hydrogen sulfide and carbon monoxide in collagen-induced platelet aggregation in patients with coronary artery disease

Purpose of research. To study the effect of hydrogen sulfide and carbon monoxide donors on collagen-induced platelet aggregation in healthy donors and patients with coronary artery disease.Introduction. There is evidence that the hydrogen sulfide (H₂S) and carbon monoxide (CO) gas transmitters play an essential role in regulating the functional activity of various organs and systems of the human body. According to previous reports, CO and H S are considered inhibitors of platelet aggregation.

Material and Methods. A total of 37 participants were examined during the study. The control group included 13 healthy donors; group of patients with coronary artery disease comprised 24 people. All examined patients received regular combination baseline therapy in accordance with modern recommendations for the treatment of coronary artery disease. Platelet aggregation activity was studied by a turbidimetric method using a two-channel laser analyzer (220 LA Biola, Russia). The degree and rate of platelet aggregation were determined in platelet-rich plasma based on the curves of light transmission and the average size of aggregates as markers of the functional activity of platelets. Collagen at a final concentration of 2 mg/mL was used to induce platelet aggregation. Sodium hydrosulfide (NaHS) was used as a hydrogen sulfide donor. Carbon monoxide-releasing molecule-II (CORM-II) was used as a carbon monoxide donor. Experimental data are presented as Me (Q₁ –Q₃). Wilcoxon T-test was used to test the homogeneity of paired or dependent samples. Values were considered tatistically significant when p was < 0.05.

Results. A hydrogen sulfide donor causes a decrease in collagen-induced platelet aggregation along the light transmission curve in both healthy donors and patients with CHD. Collagen-induced platelet aggregation along the light transmission curve is reduced by the action of the carbon monoxide donor in healthy donors, the effect is more pronounced than in patients with CHD.

Conclusion. The study showed that the effects of gas transmitters, donors of hydrogen sulfide and carbon monoxide, on the collagen-dependent platelet aggregation were unidirectional and were associated with a decrease in platelet aggregation activity in healthy donors and in patients with coronary artery disease

aggregation, gas transmitters, hydrogen sulfide, carbon monoxide

1. Gorbachev V.V. Ischemic heart disease. Moscow: Vashashaya Shkola; 2008:480 (In Russ.).

2. Medicine of emergency. Selected clinical lectures. V. 2; edit. by V.V. Nikonov, A.E. Feskov, B.S. Fedak. Donetck: ID Zaslavskiy; 2007:408 (In Russ.).

3. Barsukov A.V., Shustov S.B. Arterial hypertension. Clinical profiling and choice of therapy. Moscow: ALBI-SPb; 2014:249 (In Russ.).

4. Hartsfield C.L. Cross talk between carbon monoxide and nitric oxide. Antioxid. Redox. Signal. 2002;4(2):301–307. DOI: 10.1089/152308602753666352.

5. Li L., Hsu А., Moore P.K. Actions and interactions of nitric oxide, carbon monoxide and hydrogen sulphide in the cardiovascular system and in inflammation – a tale of three gases! Pharmacol. Ther. 2009;123(3):386– 400. DOI: 10.1016/j.pharmthera.2009.05.005.

6. Wang R. Two’s company, three’s a crowd: can H S be the third endogenous gaseous transmitter? FASEB J. 2002;16(3):1792–1798. DOI: 10.1096/fj.02-0211hyp.

7. Chirkov Yu.Yu., Belushkina N.N., Тyschuk I.A., Severina I.S. Changes in the activity of human platelet guanylatecyclase in ADP-induced aggregation. Bulletin of Еxperimental Biolоgy аnd Мedicine.1991;52(2):152–154 (In Russ.).

8. Truss N.J., Warner T.D. Gasotransmitters and platelets. Pharmacol. Ther. 2011;132(2):196–203. DOI: 10.1016/j.pharmthera.2011.07.001.

9. Petrova I.V., Trubacheva O.A., Mangataeva O.S., Suslova T.E., Kovalev I.V., Gusakova S.V. Influence of hydrogensulfide on collagen-induced aggregation of human platelets. I.M. Sechenov Russian Journal of Physiology. 2015;101(10):1191–1201 (In Russ.).

10. Kramkowski K., Leszczynska A., Mogielnicki A., Chlopicki S., Fedorowicz A., Grochal E. et al. Antithrombotic рroperties of water-soluble carbon monoxide-releasing molecules. Arterioscler. Thromb. Vasc. Biol. 2012;32(9):2149–2156. DOI: 10.1161/ATVBAHA.112.253989.