Damage, inflammation of the vascular endothelium, hypercoagulable as a risk factor for heart diseases in patients with chronic alcoholism

The study examined the markers of damage and inflammation of the vascular wall and abnormal blood coagulation in 60 patients with chronic alcoholism stage II (heavy drinkers). High-sensitive CRP (hsCRP) levels were measured by immunoturbidimetric assay with Termo Fisher reagents (Finland) and automatic biochemical analyzer Konelab 60i (Thermo Electron, Finland). Homocysteine was determined by ELISA by using Axis-Shield test system (Germany, Norway). The functional activity of von Willebrand factor (vWF) was determined by measuring ristomycin-cofactor activity (RSA) of blood plasma with Renamo test system (Russia). Level of vWF antigens were quantitively measured by ELISA with Technoclone test system (USA). Activated partial thrombin time (APTT) was determined with Platelin LS reagent (Organon Teknika Corporation). Data showed the increases in the level and activity of vWF (р<0.001) and in the levels of homocysteine (р<0.001) suggesting the damage to the vascular endothelium. The level of HsSRB increased by 3.8 times compared to healthy individuals (p<0.001) suggesting the presence of chronic indolent inflammation of the intima of the arterial wall. Decrease of aPTT (p=0.01) indicated hypercoagulability in patients with chronic alcoholism stage II. Positive correlation was found between homocysteine concentration and vWF level (r=0.46; p <0.001), homocysteine level and vWF activity (r=0.62; p<0.001), and between homocysteine and NsSRB levels (r=0.59; p<0.001). Inverse correlation was documented between APTT and vWF activity (p=0.01; r=-0.34) and homocysteine level (p=0.03; r=-0.36). In summary, hyperhomocysteinemia and increased VWF activity were accompanied by hypercoagulability in patients with chronic alcoholism stage II presented with high risk of cardiovascular diseases.

хронический алкоголизм, фактор Виллебранда, гомоцистеин, С-реактивный белок, активированное частичное тромбиновое время, chronic alcoholism, von Willebrand factor, homocysteine, C-reactive protein, activated partial thrombin time

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