PHENOTYPIC HETEROGENEITY OF CARDIAC MACROPHAGES DURING WOUND HEALING FOLLOWING MYOCARDIAL INFARCTION: PERSPECTIVES IN CLINICAL RESEARCH
https://doi.org/10.29001/2073-8552-2018-33-2-70-76
Abstract
Purpose. Myocardial regeneration is one of the most ambitious goals in prevention of adverse cardiac remodeling. Macrophages play a key role in transition from inflammatory to regenerative phase during wound healing following myocardial infarction (MI). We have accumulated data on macrophage properties ex vivo and in cell culture. However, there is no clear information about phenotypic heterogeneity of cardiac macrophages in patients with MI. The purpose of the project was to assess cardiac macrophage infiltration during wound healing following myocardial infarction in clinical settings taking into consideration experimental knowledge.
Material and Methods. The study included 41 patients with fatal MI type 1. In addition to routine analysis, macrophages infiltration was assessed by immunohistochemistry. We used CD68 as a marker for the cells of the macrophage lineage, while CD163, CD206, and stabilin-1 were considered as M2 macrophage biomarkers. Nine patients who died from noncardiovascular causes comprised the control group.
Results. The intensity of cardiac macrophage infiltration was higher during the regenerative phase than during the inflammatory phase. Results of immunohistochemical analysis demonstrated the presence of phenotypic heterogeneity of cardiac macrophages in patients with MI. We noticed that numbers of CD68+, CD163+, CD206+, and stabilin-1+ macrophages depended on MI phase.
Conclusion. Our study supports prospects for implementation of macrophage phenotyping in clinic practice. Improved understanding of phenotypic heterogeneity might become the basis of a method to predict adverse cardiac remodeling and the first step in developing myocardial regeneration target therapy.
About the Authors
A. E. Gombozhapova
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation
Russian Federation
Gombozhapova Aleksandra E. - Junior Research Worker of Emergency Cardiac Care Department, Cardiology Research Institute, TNRMC, RAS; Junior Research Worker of the Laboratory of Translational Cellular and Molecular Biomedicine at the NRTSU.
111a, Kievskaya str., Tomsk, 634012; 36, Lenin ave., Tomsk, 634050
Yu. V. Rogovskaya
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation
Russian Federation
Rogovskaya Yuliya V. - Cand. Sci. (Med.), Chief of Pathoanatomical Department, Cardiology Research Institute, TNRMC, RAS; Leading Research Officer of the Laboratory of Translational Cellular and Molecular Biomedicine, NRTSU.
111a, Kievskaya str., Tomsk, 634012; 36, Lenin ave., Tomsk, 634050
M. S. Rebenkova
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation
Russian Federation
Rebenkova Maria S. - Junior Research Worker of the Clinical Diagnostic Laboratory, Cardiology Research Institute, TNRMC, RAS; Junior Research Worker of the Laboratory of Translational Cellular and Molecular Biomedicine at the NRTSU.
111a, Kievskaya str., Tomsk, 634012; 36, Lenin ave., Tomsk, 634050
J. G. Kzhyshkowska
National Research Tomsk State University
Russian Federation
Russian Federation
Kzhyshkowska Julia G. - Dr. Sci. (Biol.), Professor, the Head of the Department of Innate Immunity and Tolerance at the Institute for Transfusion and Clinical Immunology, Medical Faculty Mannheim, University of Heidelberg; the Head of the Laboratory of Translational Cellular and Molecular Biomedicine at the NRTSU.
36, Lenin ave., Tomsk, 634050
V. V. Ryabov
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences; National Research Tomsk State University; Siberian State Medical University
Russian Federation
Russian Federation
Ryabov Vyacheslav V. - Dr. Sci. (Med.), Leading Research Officer, Chief of the Cardiac Emergency Department, Cardiology Research Institute, TNRMC, RAS; Leading Research Officer of the Laboratory of Translational Cellular and Molecular Biomedicine, NRTSU; Professor of Cardiology Department, SSMU.
111a, Kievskaya str., Tomsk, 634012; 36, Lenin ave., Tomsk, 634050; 2, Moskovsky tract, Tomsk, 6340500
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Review
For citations:
Gombozhapova A.E., Rogovskaya Yu.V., Rebenkova M.S., Kzhyshkowska J.G., Ryabov V.V. PHENOTYPIC HETEROGENEITY OF CARDIAC MACROPHAGES DURING WOUND HEALING FOLLOWING MYOCARDIAL INFARCTION: PERSPECTIVES IN CLINICAL RESEARCH. Siberian Journal of Clinical and Experimental Medicine. 2018;33(2):70-76. (In Russ.) https://doi.org/10.29001/2073-8552-2018-33-2-70-76
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